Ottaviani, Maria Francesca and Pregnolato, Massimo and Cangiotti, Michela and Fiorani, Luigi and Fattori, Alberto and Danani, Andrea (2012) Spin probe analysis of microtubules structure and formation. Archives of Biochemistry and Biophysics, 522 (1). pp. 1-8. ISSN 0003-9861
Full text not available from this repository.Abstract
Microtubules (MTs) control cell replication, material transport and motion in eukaryotic cells, but MT role in several pathologies is still unknown. These functions are related to the MT physico-chemical properties and MT formation mode starting from tubulin molecules. This study describes a new method, based on the computer aided analysis of the electron paramagnetic resonance (EPR) spectra of selected spin probes to obtain structural and dynamical information on tubulins and MTs and the kinetics of MTs formation promoted by guanosine-5'-triphosphate (GTP). It was found that tubulin and MTs avoid radical quenching caused by ethylene glycol tetraacetic acid (EGTA). MT formation showed different kinetics as a function of tubulin concentration. At 5 mg/mL of tubulin, MTs were formed in 8 min. These results are also useful for getting information on MT-drug interactions.
Item Type: | Scientific journal article, Newspaper article or Magazine article |
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Subjects: | Physical sciences > Physics > Chemical physics |
Department/unit: | Dipartimento tecnologie innovative > Istituto di ingegneria meccanica e tecnologia dei materiali |
Depositing User: | Andrea Danani |
Date Deposited: | 06 Mar 2015 06:58 |
Last Modified: | 08 Jul 2016 11:48 |
URI: | http://repository.supsi.ch/id/eprint/6243 |
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