High-resolution optical identification of Aedes albopictus and Aedes aegypti eggs

Anicic, Nikoleta and Marina, Carlos F. and Fouque, Florence and Velayudhan, Raman and Flacio, Eleonora (2022) High-resolution optical identification of Aedes albopictus and Aedes aegypti eggs. In: E-SOVE 2022, 11-14 October 2022, Sofia, Bulgaria.

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Invasive mosquito species of the genus Aedes pose a risk to public health due to their ability to transmit viral diseases (e.g. Dengue, Chickungunya, Zika), which is why there is a need to monitor and control these species. The objective of this project was to develop a high-resolution optical instrument to identify differences in the chorion structure of eggs of the two main vector species (Aedes albopictus and Aedes aegypti). This system would allow a rapid analysis of the samples collected in the field and a quantification of the abundance of the two species when both are present, in order to improve arboviral disease surveillance. In the first part of the project, researchers from the Centro Regional de Investigación en Salud Pública/Instituto Nacional de Salud Pública (CRISP/INSP) collected Aedes eggs in Mexico using ovitraps, while the Vector Ecology Unit (SUPSI-DACD-Institute of Microbiology) performed the optical analysis of Aedes eggs with a high-resolution microscope. The results obtained were confirmed by molecular analysis. The entire project was funded by the Special Programme for Research and Training in Tropical Diseases (TDR), hosted at the World Health Organisation (WHO) and the Department of Neglected Tropical Diseases (NTD/WHO).

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