Real-time elastography using an external reference material: test-retest reliability on healthy achilles tendon

Schneebeli, Alessandro and Del Grande, Filippo and Vincenzo, Gabriele and Cescon, Corrado and Clijsen, Ron and Biordi, Fulvio and Barbero, Marco (2016) Real-time elastography using an external reference material: test-retest reliability on healthy achilles tendon. In: IFOMPT Conference 2016 IFOMPT Conference 2016, 04.07.2016-08.07.2016, Glasgow, United Kingdom. (Submitted)

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Abstract

Background. Real-time elastography is a recent ultrasound-based technique which allows a qualitative assessment of tissue elasticity. Light and transverse tissue compression with an ultrasound probe is applied to a surface producing a displacement within the tissue, which is less pronounced in harder than in softer materials. Tissue displacement is calculated by comparing b-mode image pairs before and after the compression. Elasticity pattern are represented on the ultrasound monitor as a colour scale superimposed on a B-mode image. Purpose.. To establish the test–retest reliability of sonoelastography (SE) on healthy Achilles tendon in contracted and relaxed states using an external reference system. Materials and Methods. Forty-eight Achilles tendons from 24 healthy volunteers were assessed using ultrasound and real-time SE with an external reference material. Tendons were analyzed under relaxed and contracted states. Strain ratios between tendons and reference material were calculated. The intraclass correlation coefficient (ICC2.k) and Bland-Altman plot were used to assess test–retest reliability. Results. Reliability of SE measurements in relaxed state ranged from high to very high with an ICC2.k of 0.84 (95%CI: 0.64–0.92) for reference material, 0.91 (95%CI: 0.83–0.95) for Achilles tendon and 0.95 (95%CI: 0.91–0.97) for Kager fat pad (KFP). The ICC2.k value for skin was 0.30 (95%CI: –0.26 to 0.61). Reliability for measurements in the contracted state ranged from high to very high with an ICC2.k of 0.93 (95%CI: 0.87–0.96) for reference material, 0.72 (95%CI: 0.50–0.84) for skin, 0.93 (95%CI: 0.87–0.96) for Achilles tendon and 0.81 (95%CI: 0.66–0.89) for KFP. Reliability of the strain ratio (Tendon/Reference) in the relaxed state was high with an ICC2.k of 0.87 (95%CI: 0.75–0.93), and in the contracted state, it was very high with an ICC2.k of 0.94 (95%CI: 0.90–0.97). Conclusion. SE using an external reference material is a reliable and simple technique for elasticity assessment of healthy Achilles tendon. The use of an external material as a reference along with strain ratios could provide a quantitative measure of elasticity. Implications. Keyword. Sonoelastography; Achilles tendon; reliability Funding acknowledgements. We especially thank the Thim van der Laan Foundation, Landquart, Switzerland, for financial support. Ethics approval. The study was approved by the Ethics Committee of Canton Ticino, Switzerland.

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