Buetti-Dinh, Antoine and Roman, Samuele and Di Nezio, Francesco and Fava, Tommaso and Paganini, Daniele and Storelli, Nicola (2022) Applied bioinformatics in alpine microbial hydrological systems: Transcriptomic comparison between environment and lab. In: 18th International Symposium on Microbial Ecology, 14-19.08.2022, Lausanne.
Full text not available from this repository.Abstract
The Swiss alpine environments are poorly described from a microbiological perspective. OMICs technologies allow to explore a multitude of environments where microbial life adapted in stunning ways to cope with the conditions that characterize the Swiss alpine environment. Here we present an overview of our research activities that employ new generation sequencing, in combinations with actual bioinformatic workflows and high-performance computing for reverse engineering biological networks. Lake Cadagno, in the Piora valley in Ticino, is a meromictic lake organized in three different layers in which distinct ecosystems interact. The middle layer is characterized by anoxygenic photosynthesis driven by three major bacterial species, which have adopted different strategies to carry out their photosynthetic activities. The complex interaction between them abuts in a process of bioconvection that we attempt to dissect using metatranscriptomics together with other environmental parameters and physiological analyses. Another alpine ecosystem characterized by a naturally occurring abundance of heavy metals is also a focus of our interests. Near the Greina plateau in the Camadra valley in Ticino, a green-coloured water spring streams off the mountain cliffs. The highly unusual chemical composition of this environment was of particular interest especially due to the presence of a thick biofilm, mainly composed of cyanobacteria. Here, in addition to a deep geochemical profiling and microscopic analysis, we carried out a metagenomic study to identify the organisms that constitute the biofilm, as well as assembling a metagenome to shed light on the genes that have been selected to allow adaptation in such exceptional environment.
| Item Type: | Article in conference proceedings or Presentation at a conference (Poster) |
|---|---|
| Subjects: | Biological Sciences > Biology > Ecology Biological Sciences > Genetics > Molecular genetics > Transcriptomics Biological Sciences > Microbiology |
| Department/unit: | Dipartimento ambiente costruzioni e design > Istituto microbiologia |
| Depositing User: | Nicola Storelli |
| Date Deposited: | 16 Mar 2023 08:09 |
| Last Modified: | 16 Mar 2023 08:10 |
| URI: | http://repository.supsi.ch/id/eprint/13860 |
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