Stoffel, Nicole U. and Zeder, Christophe and Fort, Eloise and Swinkels, Dorine W. and Zimmermann, Michael B. and Moretti, Diego (2017) Prediction of human iron bioavailability using rapid c-ELISAs for human plasma hepcidin. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 55 (8). pp. 1186-1192.
Full text not available from this repository.Abstract
Background: Hepcidin is the central systemic regulator of iron metabolism, but its quantification in biological fluids is challenging. Rapid, accurate and user-friendly methods are needed. Our aim was to assess the ability of hepcidin as measured by three different c-ELISA assays to predict iron bioavailability in humans. Methods: The three assays used were commercially available DRG and Peninsula assays and the c-ELISA method performed at Radboud University Medical Centre, Nijmegen, The Netherlands (Hepcidinanalysis. com), validated by comparative measurements with time-of-flight mass spectrometry. We analyzed plasma samples (n = 37) selected to represent a broad range of hepcidin concentrations from a subgroup of healthy, iron-depleted women in a study assessing fractional absorption from iron supplements. Results: In single regressions, all three c-ELISA assays were predictors of fractional iron absorption: R-2 = 0.363 (DRG), R-2 = 0.281 (Peninsula) and R-2 = 0.327 (Hepcidinanalysis. com). In multiple regressions, models including hepcidin measured with either DRG-, Peninsula or Hepcidinanalysis. com explained 55.7%, 44.5% and 52.5% of variance in fractional absorption, and hepcidin was a strong predictor of fractional absorption irrespective of the hepcidin assays used. However, we found significant differences in absolute values for hepcidin between different methods. Both the DRG assay's (y = 0.61x + 0.87; R-2 = 0.873) and the Peninsula assay's measurements (y = 1.88x + 0.62; R-2 = 0.770) were correlated with Hepcidinanalysis. com. Conclusions: The biological variability in plasma hepcidin, (inter-sample CV) was 5-10-fold higher for both the Peninsula and DRG assay than the analytical variably (inter-run within-sample CV) suggesting substantial discriminatory power to distinguish biological hepcidin variation. Between methods, prediction of iron bioavailability in generally healthy iron depleted subjects appears comparable.
| Item Type: | Scientific journal article, Newspaper article or Magazine article |
|---|---|
| Subjects: | Subjects Allied to Medicine > Nutrition > Dietetics Subjects Allied to Medicine > Nutrition > Nutrition not elsewhere classified |
| Department/unit: | Fernfachhochschule Schweiz |
| Depositing User: | Diego Moretti |
| Date Deposited: | 24 Sep 2019 04:56 |
| Last Modified: | 24 Sep 2019 04:56 |
| URI: | http://repository.supsi.ch/id/eprint/10785 |
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