Presence of antibiotic resistance genes in Lugano Lake and some tributary rivers.

Mauri, Federica and Demarta Aeschbacher, Antonella (2018) Presence of antibiotic resistance genes in Lugano Lake and some tributary rivers. UNSPECIFIED. In: Halting Antimicrobial Resistance Dissemination in Aquatic Environments (HEARD2018), 16-19 Settembre 2018, Ascona. (Unpublished)

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Abstract

The aim of our work was to observe if anthropic activities could influence the bacterial populations naturally present in water environments in terms of antibiotic resistance. To reach our objective we monitored, for two years (2016 and 2017), the presence of antibiotic resistance genes in water samples collected from Lake Lugano and some tributary rivers. We analysed the samples by PCR to investigate the presence or absence of integrase gene and of seven genes that confers resistance to the major classes of antibiotics used in clinical and veterinary (β-lactams, tetracycline, quinolones, sulphonamides and macrolides). After the qualitative screening, we quantified resistance genes using qPCR. We found at least five quantifiable genes sulII, qnrS, tetA, blaTEM and ermB. The amount of resistance genes was compared with the 16S. There was a global increase of resistance genes in 2017 especially in the river influenced by wastewater treatment plant and in the lake.

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